Holbeach United’s FA Vase run was ended in the second round at Long Eaton.
Stacy Cartwright had given the Tigers the lead in Derbyshire with an early strike.
However, the hosts went into the interval 2-1 up courtesy of Rob McCormick’s brace.
Tom Roberts’ side pulled level after the re-start as Jordan Thomas came off the bench and got his name on the scoresheet.
However, the killer blow was struck by Shaun Rickford, earning Long Eaton a 3-2 success and a place in the third round.
The Tigers return to United Counties League Premier Division action on Wednesday night.
They will travel to Jubilee Park to face basement side Huntingdon Town.
The contest will kick off at 7.45pm.