Joint-manager John Chand has warned Holbeach United to “take nothing for granted”.
The Tigers take the first step on what they hope will be the road to Wembley in the FA Vase when Blackstones head to Carter’s Park.
Premier Division title contenders Holbeach are hot favourites for the second qualifying round tie against the UCL Division One side.
Chand said: “We’ve had a good look at Blackstones.
“People will see our league form and their position and say this is a game which we should win.
“If we have aspirations of going anywhere in the FA Vase then we’ve got to win.
“But it will be important to approach this game with the right mindset and to take nothing for granted.
“In each game we tell the players that they have got to do the right things.
“We want them to be ruthless when we are on top in games.”
Matt Warfield suffered a recurrence of an injury problem in the midweek cup win while Tommy Treacher was taken off at half-time as a precaution.
Both players should be available this weekend but Lee Beeson is serving a three-match suspension.
Chand, meanwhile, says working with joint-manager Graham Drury is “brilliant”.
He added: “I didn’t know how I would fit into a partnership or even as a number two because I will always have my own views.
“It has worked well so far and we speak a number of times every day.
“We want to win and it helps to be able to share the duties.
“We have different contacts around the leagues and that helps too.”