Spalding’s Winsover Road will close to traffic from 10pm on Saturday to 9am on Sunday while workmen demolish a signal box.
Work starts on the day the town is predicted to face gridlock as railway barriers at Winsover Road and other level crossings go down to allow more than 100 trains through.
Trains will be diverted over five weekends as Network Rail carries out multiple improvements to the East Coast main line.
Work on stripping the signal box has already begun and letters have gone out to warn residents about possible noise from the demolition works.
Demolition will be carried out while trains are not running.
Network Rail told residents: “We need to demolish Spalding signal box as this area of track has had a new signalling system recently installed which is now run from Lincoln signal box.
“The existing signal box is now redundant and needs demolishing to free up (the) area and negate any future maintenance to ensure it is safe and secure.
“The work will involve midweek day shifts to soft strip the internals of the signal box (remove the equipment, etc) and working within a full road closure on Saturday night and Sunday day whilst trains are not running, using excavators to demolish the building, loading the redundant materials into lorries/skips for removal from site.”
Lincolnsire County Council highways said the work was due to take place last weekend, but Network Rail rescheduled it.
Deeping St James residents won the battle to save their demolished signal box – which is due to be rebuilt on another site - but the only move to save Spalding’s was from a resident who wanted to turn it into a dolls’ house workshop. Network Rail doesn’t need planning consent for demolition as it has permitted development rights.